![PCR question: Do I have primer dimers (lane 3 & 5)? Hi lab rats, I performed a gradient PCR today with successful amplification at lower temps for two sets of primers (in PCR question: Do I have primer dimers (lane 3 & 5)? Hi lab rats, I performed a gradient PCR today with successful amplification at lower temps for two sets of primers (in](https://i.redd.it/l1g5gz64ae431.jpg)
PCR question: Do I have primer dimers (lane 3 & 5)? Hi lab rats, I performed a gradient PCR today with successful amplification at lower temps for two sets of primers (in
![Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR | Experimental & Molecular Medicine Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR | Experimental & Molecular Medicine](https://media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs12276-020-0452-7/MediaObjects/12276_2020_452_Fig2_HTML.png)
Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR | Experimental & Molecular Medicine
![Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus | BMC Microbiology | Full Text Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus | BMC Microbiology | Full Text](https://media.springernature.com/lw685/springer-static/image/art%3A10.1186%2F1471-2180-14-10/MediaObjects/12866_2013_Article_2447_Fig2_HTML.jpg)
Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus | BMC Microbiology | Full Text
![How do I detect primer dimers in an electrophoresis gel? and how to avoid them in a PCR reaction? | ResearchGate How do I detect primer dimers in an electrophoresis gel? and how to avoid them in a PCR reaction? | ResearchGate](https://www.researchgate.net/profile/Guillermo-Uceda-Gomez/post/How_do_I_detect_primer_dimers_in_an_electrophoresis_gel_and_how_to_avoid_them_in_a_PCR_reaction/attachment/5eb14b56c005cf0001886185/AS%3A887791776956416%401588677462190/download/01_27_04_2020.jpg)
How do I detect primer dimers in an electrophoresis gel? and how to avoid them in a PCR reaction? | ResearchGate
Preetha was practising PCR hands on in her laboratory. She successfully isolated the gene of interest GOI, however the quantity was too low. She desired to amplify the sequence. She conducted a
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